cell culture conditions murine breast cancer cells emt 6 Search Results


emt6 murine breast cancer cells  (ATCC)
99
ATCC emt6 murine breast cancer cells
The in vitro uptake of [ 18 F]FB-Tz in (A) <t>EMT6</t> and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).
Emt6 Murine Breast Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell culture murine cancer cell lines  (ATCC)
99
ATCC cell culture murine cancer cell lines
The in vitro uptake of [ 18 F]FB-Tz in (A) <t>EMT6</t> and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).
Cell Culture Murine Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc number pta 2755  (ATCC)
97
ATCC atcc number pta 2755
The in vitro uptake of [ 18 F]FB-Tz in (A) <t>EMT6</t> and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).
Atcc Number Pta 2755, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine mammary adenocarcinoma cell lines  (ATCC)
95
ATCC murine mammary adenocarcinoma cell lines
The in vitro uptake of [ 18 F]FB-Tz in (A) <t>EMT6</t> and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).
Murine Mammary Adenocarcinoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4t1 emt6 jc murine mammary carcinoma cells  (Charles River Laboratories)
86
Charles River Laboratories 4t1 emt6 jc murine mammary carcinoma cells
The in vitro uptake of [ 18 F]FB-Tz in (A) <t>EMT6</t> and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).
4t1 Emt6 Jc Murine Mammary Carcinoma Cells, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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emt6 murine mammary carcinoma cells  (Thermo Fisher)
99
Thermo Fisher emt6 murine mammary carcinoma cells
The in vitro uptake of [ 18 F]FB-Tz in (A) <t>EMT6</t> and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).
Emt6 Murine Mammary Carcinoma Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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emt6/p murine breast carcinoma cell line  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures emt6/p murine breast carcinoma cell line
The in vitro uptake of [ 18 F]FB-Tz in (A) <t>EMT6</t> and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).
Emt6/P Murine Breast Carcinoma Cell Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sw480 ancer cell lines murine mammary carcinoma cell line emt 6  (ATCC)
99
ATCC sw480 ancer cell lines murine mammary carcinoma cell line emt 6
The in vitro uptake of [ 18 F]FB-Tz in (A) <t>EMT6</t> and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).
Sw480 Ancer Cell Lines Murine Mammary Carcinoma Cell Line Emt 6, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The in vitro uptake of [ 18 F]FB-Tz in (A) EMT6 and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).

Journal: Rsc Advances

Article Title: Detecting hypoxia in vitro using 18 F-pretargeted IEDDA “click” chemistry in live cells †

doi: 10.1039/d1ra02482e

Figure Lengend Snippet: The in vitro uptake of [ 18 F]FB-Tz in (A) EMT6 and (B) HCT116 cells. Cells were treated with 8 (0.01–10 μM) and cultured under normoxia or hypoxia (0.1% O 2 ) conditions for 24 h prior to radioactive uptake experiment: 0.74 MBq per well of [ 18 F]FB-Tz was added to cells and incubated for 60 min. (C) Time course of [ 18 F]FB-Tz uptake in EMT6 cells in vitro . Cells were treated with 8 (10 μM) for 24 h under hypoxia before incubation with 0.74 MBq per well of [ 18 F]FB-Tz for 5, 10, 15, 30, 45 and 60 min. (D) Radioactive uptake of [ 18 F]FMISO (1.48 MBq per well) for 0 min (pulse) or 3 h under normoxia or hypoxia conditions in EMT6 and HCT116. Data were expressed as a percentage of total radioactivity incorporated into cells, normalized for total cellular protein. Mean values ± SD ( n = 6; * P < 0.05, ** P < 0.01 and **** P < 0.0001).

Article Snippet: EMT6 murine breast cancer cells were a kind gift from Prof. Sofia Pascu (University of Bath, UK) and were grown in Waymouth medium (Thermo Fisher Scientific); HCT116 human colorectal cancer cells (ATCC) were grown in Dulbecco Modified Eagle Medium (DMEM, Sigma-Aldrich).

Techniques: In Vitro, Cell Culture, Incubation, Radioactivity